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start dna platinum taq  (Thermo Fisher)


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    Thermo Fisher start dna platinum taq
    Start Dna Platinum Taq, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 462935 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/start dna platinum taq/product/Thermo Fisher
    Average 99 stars, based on 462935 article reviews
    start dna platinum taq - by Bioz Stars, 2026-03
    99/100 stars

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    Optimization of one-step RT-qPCR reaction conditions. Various dilutions of Platinum II Taq Hot-Start DNA Polymerase buffer were first optimized relative to the use of 1 U SuperScript IV Reverse Transcriptase and 1 U Platinum II Taq Hot-Start DNA Polymerase per well. Reactions using the RNA UltraSense (RNA-US) kit served as a positive control. (A) Representative amplification plots and (B) mean Ct values with standard error of measurement (SEM) obtained from 2 independent experiments, each with 2 technical replicates. Statistical analysis: repeated-measures one-way ANOVA (RM-ANOVA); n = sample number; ***P < 0.001. Outliers deviating by >4 Ct from the dataset mean were excluded.

    Journal: MethodsX

    Article Title: Development of an in-house, one-step RT-qPCR mix and optimized MS2 detection primers for hepatitis A virus and norovirus detection in berries 1

    doi: 10.1016/j.mex.2025.103703

    Figure Lengend Snippet: Optimization of one-step RT-qPCR reaction conditions. Various dilutions of Platinum II Taq Hot-Start DNA Polymerase buffer were first optimized relative to the use of 1 U SuperScript IV Reverse Transcriptase and 1 U Platinum II Taq Hot-Start DNA Polymerase per well. Reactions using the RNA UltraSense (RNA-US) kit served as a positive control. (A) Representative amplification plots and (B) mean Ct values with standard error of measurement (SEM) obtained from 2 independent experiments, each with 2 technical replicates. Statistical analysis: repeated-measures one-way ANOVA (RM-ANOVA); n = sample number; ***P < 0.001. Outliers deviating by >4 Ct from the dataset mean were excluded.

    Article Snippet: The same reaction conditions were used for commercial kits and for in-house mix with SuperScript IV reverse transcriptase and Platinum II Taq Hot-Start DNA polymerase (Thermo Fisher Scientific).

    Techniques: Quantitative RT-PCR, Reverse Transcription, Positive Control, Amplification